BIOCHEMICAL STUDIES ON HUMAN SALIVARY PROTEINS II. PRELIMINARY CHARACTERIZATION OF A FRACTION WITH HYPOCALCEMIC AND LEUKOCYTOSIS-PROMOTING ACTIVITIES IN THE DIGEST OF SALIVA-PAROTIN-A BY PRONASE P

Abstract

Fractionation of the digest of saliva-parotin-A by Pronase P was carried out by gel filtration on Sephadex G-25 column to give five fractions (P-1--V), using 1M acetic acid as eluant. Of five fractions, only P-I containing peptides of fairly large molecular weights showed potent hypocalcemic and leukocytosis-promoting activities in rabbits. Electrophoretical behavior and amino acid pattern of P-I were quite different from those of saliva-parotin-A.