Abstract
Effective doses of synthetic LH-RF for induction of ovulation at various stages, except the stage of estrus, of 4-day cyclic rats and mice were investigated. In rats injected subcutaneously at 4p.m., the maximal effective doses were 6.4μg on mtestrus, 0.8μg on diestrus, and 0.2μg on proestrus (proestrus rats were pretreated with pentobarbital). In mice the corresponding doses were 0.05μg on diestrus, and 0.02μg on proestrus. On metestrus, however, most of the mice ovulated by 0.4μg, while even larger doses up to 1.6μg failed to elicit ovulation in the others.
