Endocrinologia Japonica
Online ISSN : 2185-6370
Print ISSN : 0013-7219
ISSN-L : 0013-7219
Application of a Marker Dye on Sephadex LH-20 Column Chromatography in Radioimmuno-Assay of Plasma Aldosterone
SHUICHI ICHIKAWAKAZUHIKO MURATARYOICHI SHIGIYA
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1974 Volume 21 Issue 4 Pages 361-365

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Abstract
Aldosterone in 2 ml of plasma was determined by radioimmunoassay. A small modified 10 ml pipette column of LH-20 (0.8×15 cm) was used for separation and 1, 4-diaminoanthraquinone was employed as a marker dye. The application of the small modified column and the marker dye made the separation easy, rapid and accurate. The recovery, precision, accuracy, sensitivity and specificity of the method were satisfactory. This method was considered to be convenient enough for clinical use.
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© The Japan Endocrine Society
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