Abstract
Androsterone, etiocholanolone, pregnanetriol, dehydroepiandrosterone, pregnanediol, tetrahydrocortisol, 5-pregnenolone and 11-β-OH-androsterone were incubated with β-glucuronidase preparations (Helix pomatia, bovine live and E. coli) for 96 hrs at 37°C. After extraction and silvlation they were gas-chromatographed. The first 3 steroids were left practically intact. The least decomposition of the last 5 steroids occurred with the liver enzyme. Testosterone and 11-ketoandrosterone without the enzymes showed 74 and 35% recoveries. Cortisol and tetrahydrocortisol, incubated with the first two enzymes for 18hrs at 37° and 48°. showed nearly 100% recoveries. The recoveries of 17-OHCS in urines (pH 7.8-8.8), stored for 7 days, was 80% at 20°-25°C and 55% and 55% at 25°-30°C. The same samples, brought to pH 1.8-2.8 with NaHSO4 before the storage, showed a 100% recovery.
