Abstract
The effect of bonito insulin on insulin release was examined in the monolayer culture of rat pancreatic β-cells. The β-cells were preincubated for 5 to 20hr with or without a small dose (100μU/ml) of bonito insulin in the medium containing 100mg% glucose. And then, they were incubated in 300mg% glucose alone or together with bonito insulin for 5hr. There was no significant difference between the IRI release from these n-cells with or without bonito insulin. The concentration of bonito insulin was augmented from 100μU/ml to 500, 1, 000 and 2, 000μU/ml. A significant inhibitory effect on the glucose-induced insulin release was observed only after the preincubation for 20hr with 2, 000μU/ml of bonito insulin.
