Abstract
A simultaneous competitive enzyme immunoassay (SICEIA) for hCG was developed using β-D-galactosidase (β-Gal) as a labelled enzyme and anti-hCG antibody coatedsheep red blood cells (SRBC) as a solid phase. In this report, a new coupling agent, MCAE, was used to couple β-Gal with hCG. The sensitivity was improved to the degree of 2.5mIU/ml, equal to that of RIA. The present procedure was safer and rapider than RIA. The value of hCG in urine by our procedure had good correlation with that by RIA.
