Nuclei and DNA-Binding Form of the Steroid Receptor Complex in the Liver and the Ventral Prostate of Rats

Abstract

Repeated treatments of the liver cytosol prelabeled with ³H-dexamethasone with DNA-cellulose followed by treatments with purified liver nuclei enabled us to assess the amount of two forms of dexamethasone-receptor complex, one binds only to nuclei and the other binds to DNA and nuclei. The comparable amount of these two forms of the receptor complex was detected in the dexamethasone-labeled liver cytosol, while only one form which binds only to nuclei was found in the liver cytosol labeled with antiglucocorticoid, cortexolone. The significant amount of receptor complex which binds to DNA and nuclei was not present in the cytosol from the ventral prostate preincubated with 3H-dihydrotestosterone.