Abstract
Combination of two thyroglobulin monoclonal antibodies (monoAbs) recognizing epitopes which are rarely recognized by an antibody enabled us to develop a rapid one-step enzyme immunoassay of serum Tg. Of 87 monoclonal antibodies, 20 were selected for the purpose. The method is a sandwich technique employing a monoAb covering microplate and horse-radish peroxidase monoAb conjugate. A combination of monoAb 7A7A solid phase and 31A2E for the conjugate gave the best results. The assay takes60min and the minimal detectable amount is 2ng/ml. Intraassay variation is from 4 to 7%. Interassay variatoin is 5 to 12%. The recovery rate for Tg added to normal sera is between 89 and 111%.
The correlation coefficient with the polyclonal antibody method in Tg hemagglutination negative sera is0.98.
The presence of autoantibody in sera up to 10×24hemagglutination titer does not affect the recovery rate to a statistically significant extent.
