Effects of BETA-Hydroxy Butyric Acid on Insulin Binding to its Receptor and on Autophosphorylation of the Receptor

Abstract

We examined the effects of beta-hydroxy butyric acid (B-OH-butyrate) on insulin binding to its receptor and on autophosphorylation of the receptor in vitro. The affinity of isolated rat adipocyte insulin receptors was significantly increased by B-OH-butyrate at neutral pH. Similar results were obtained with soluble insulin receptors prepared from hepatocytes. Autophosphorylation of insulin receptor, however, was not affected by OH-butyrate at neutral pH
These results seggestd insulin resistance in ketoacidosis is not caused by an interaction between B-OH-butyrate and the insulin receptor.