Nucleotide Sequences of cDNA Clones, pSv-1 and pSv-2, Hybridizing to Androgen-Stimulated mRNAs in Rat Seminal Vesicles

Abstract

In order to further characterize the cDNA clones, pSv-1 and pSv-2, which had been newly isolated from a cDNA library of intact rat seminal vesicles as clones hybridizing to androgen-stimulated mRNAs of approximately 1, 500 and 3, 500 nucleotides in length, respectively, the whole nucleotide sequences were determined. The pSv-1 and pSv-2 were 1135 and 1819 nucleotides in length, respectively, and seemed not to contain entire sequences corresponding to the mRNAs. When the 0.7 kb Hindlll fragments from pSv-1 and the 1 kb fragments from pSv-2 were used to probe rat genomic DNA that had been digested with four restriction enzymes, the Southern blots suggested the existence of multiple genes related to pSv-1 and a single gene related to pSv-2. These results suggest that pSv-1 and pSv-2 provide useful probes not only for further characterization of products encoded by the mRNAs, but also for the study on the physiological roles of androgen-dependent gene expression in rat seminal vesicles.