Abstract
Saliva-parotin-A could not be purified by fractional precipitation with trichloroacetic acid or ammonium sulfate nor by absorption with oxycellulose. In every case, the yield and the biological activities were distributed among various fractions.
By means of fractionation with ethanol in neutral solution, the effective substance was precipitated below 60% of ethanol concentration. The effective substance was concentrated to the bottom of tubes after ultracentrifugation for 5 hrs. at 54, 000 r. p.m.
Saliva-parotin-A was purified by successive applications of fractionation with ethanol and ultracentrifugation.
