CORRELATION BETWEEN GLUCOSE BASAL UPTAKE AND INSULIN EFFECT IN RAT-DIAPHRAGM METHOD
1960 Volume 7 Issue 2 Pages 109-120
Details
1960 Volume 7 Issue 2 Pages 109-120
1. Relation bctween basal glucose uptake and the so-called insulin effect obtained by the rat-diaphragm method was investigated, and a linear correlation was confirmed within a definite range.
2. Change was produced in the properties of the correlation line by variations in the concentration of glucose or of insulin in the medium, and it was found that correlation lines were dislocated in a radiating form with the center on a definite point on the ordinate in the case of change in glucose concentration, and on the abscissa in the case of change in the insulin concentration. Attempt was made to use the slope of the correlation line as the metameter of log-dose response curve for insulin determination, and the following formulas were found for the regression line: s=0.212, b=+1.345, λ=0.158.
3. Change in glucose uptake ability elicited by change in diet composition or by insulin pretreatment took place along the correlation line, whereas variation due to the procedure of preparation of the diaphragm or to that of the determination of glucose occurs independently of the correlation line.
