A Rapid and Easy Electrophoretic Method for Detecting Biochemical Loci of Rat (Rattus norvegices)

Abstract

Methods for electrophoresis for the analysis of biochemical marker genes, which are used widely for genetic monitoring of inbred straines of rats, have been complicated by the variation of the gel and electrode buffer and electrophoretic conditions with the enzyme or the protein to be examined. To simplify the methods, we performed electrophoresis under fixed conditions of 200 V and 30 min using cellulose acetate membrane as the gel and veronal solution as the gel and electrode buffer. Good results were obtained concerning 12 loci, namely, Amy1, Cs1, Esl, Es2, Es3, Es4, Fh1, Gc, Hbb, Ldr1, Mup1, and Svp1. This method was applied to 8 inbred strains of rats and confirmed to be practical.