Experimental Animals
Online ISSN : 1881-7122
Print ISSN : 1341-1357
ISSN-L : 0007-5124
Note
Typing of X Chromosomes Bearing Tabby Allele in Mouse Preimplantation Embryos by Detection of a Microsatellite Marker
Ruby P.A. VALDIVIATetsuo KUNIEDAMasahira HATTORIYutaka TOYODA
著者情報
キーワード: microsatellite, Tabby, XO
ジャーナル フリー

1996 年 45 巻 4 号 p. 395-398

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Tabby (Ta) is a semidominat allele of the locus on the mouse X chromosome, which causes a characteristic coat pattern and developmental defects in endocrinic glands. To establish a method for identifying the X chromosome bearing the Ta allele in early preimplantation embryos, we performed PCR amplification of an X chromosomal microsatellite marker locus from preimplantation embryos obtained from mating between XO female and XY male mice. The microsatellite marker locus was shown to be polymorphic between X chromosomes bearing the Ta and wild-type alleles. The amplification of the marker locus from early preimplantation embryos demonstrated that the Ta locus can be correctly typed by this method from embryos as little as 2 cells.

著者関連情報
© 1996 Japanese Association for Laboratory Animal Science
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