抄録
In order to evaluate the availability of plasma amino acid (AA) concentration as a parameter to determine AA requirements of rats within a short period, the response of the plasma AA concentration to changes in dietary AA levels was studied. In Experiment 1, to clarify whether the plasma AA concentration responded to changes in dietary AA levels and how long the response of the plasma AA concentration was maintained, 10 male rats were fed a casein diet for a 7 day period, and then the food was changed to AA diets. The serine (Ser), glycine (Gly) and threonine (Thr) levels were decreased from 120, 90 to 60% of the casein diet at 7-day intervals successively. Blood samples were taken at 2, 4 and 7 days after changing the diet, from the vena caudalis by vein puncture. In all cases, the plasma AA concentration responded to changes in dietary AA within 2 days, and the response was maintained until the 7th day. Experiment 2 was conducted to confirm that the plasma concentration of each AA responded to the changes in dietary AA levels at the first day and the responses of the plasma concentration of AA were maintained until the 4th day after changing the dietary AA levels, when the combination of metabolically unrelated AA was added. Five rats in A groups were fed diets with added arginine + Thr, histidine + methionine, lysine or isoleucine + Ser, and five rats in B groups were fed diets with added Gly + valine, leucine + phenylalanine or Thr + tyrosine+valine for a 4-day period successively. Blood samples were taken at 1 and 4 days after changing diets. Although the plasma concentrations of histidine, leucine, methionine, phenylalanine and Ser did not respond to changes in dietary AA levels, those of other AA responded at 1 day and the responses were maintained until the 4th day.
