Experimental Animals
Online ISSN : 1881-7122
Print ISSN : 1341-1357
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Cryopreservation and Transport of Mouse Spermatozoa at -79°C
Masanori OKAMOTONaomi NAKAGATAYutaka TOYODA
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Volume 50 (2001) Issue 1 Pages 83-86

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Abstract

In the present study, 2 experiments were carried out. In experiment 1, mouse spermatozoa were frozen and stored in an ultra-low temperature freezer maintained at -79°C, from 1 week to 8 months. In vitro fertilization rates of the frozen-thawed sperm after 1 week and 4 months of storage were high at 71 and 71%, respectively. These values did not differ significantly from the value (73%) of the control stored at -196°C. In contrast, the 8-month storage rate was significantly lower at 51%. In experiment 2, frozen spermatozoa were transported in a Styrofoam box packed in dry ice from Hokkaido to Tokyo. In vitro fertilization rate of frozen-thawed sperm after transport at -79°C was high at 88%, which was not significantly different from that (84%) of the transported control at -190°C. After transferring two-cell embryos derived from frozen spermatozoa to recipients, 37-62% of the embryos developed into offspring in both experiments. These results indicate that mouse spermatozoa can survive cryopreservation in an ultra-low temperature freezer (-79 °C) for up to 4 months and transport at -79°C.

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© 2001 Japanese Association for Laboratory Animal Science
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