Detection of B Virus Infection in Cynomolgus Monkeys by ELISA Using Simian Agent 8 as Alternative Antigen

Jun-ichiro TAKANO; Toyoko NARITA; Koji FUJIMOTO; Ryozaburo MUKAI; Akio YAMADA

doi:10.1538/expanim.50.345

Abstract

The use of simian agent 8 (SA8) as an antigen for B virus (BV) antibody detection was evaluated in cynomolgus monkeys. Seventy-two sera judged as positive using BV antigen were all positive when the SA8 antigen was used. Out of 28 BV-negative sera 2 were positive against the SA8 antigen and one serum was classified as indeterminate. The present data indicates that detection of BV antibody can be achieved accurately and safely by enzyme-linked immunosorbent assay (ELISA) using SA8 antigen.

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