2001 Volume 50 Issue 5 Pages 441-443
The temporary preservation of oocytes without freezing would be useful for some experiments. ICR mouse oocytes were kept in a preservation medium under mineral oil for 1, 2, 3, 4 or 7 days at 5°C, and 1 or 2 days at 37°C. In vitro fertilization was attempted on oocytes rinsed with TYH medium after preservation. More than 70% of morphologically normal oocytes were recovered from each preservation group. Fertilization rates of oocytes preserved for 1, 2, 3, 4 or 7 days at 5°C were 69.9, 66.5, 45.3, 26.7 and 8.8% respectively. Fertilization rates of oocytes preserved for 1 or 2 days at 37°C were 9.6 and 1.6%, respectively. Preservation of oocytes at 5°C has some capability as a method of short-term storage without freezing.
Bulletin of the Experimental Animals
Proceedings of The Japanese Association of Animal Models for Human Diseases
Proceedings of The Japanese Society of Animal Models for Human Diseases
