New PCR-RFLP Analysis for the Mouse Tnfsf6^gld Gene Caused by a Point Mutation in the Tnfsf6 [Tumor Necrosis Factor (Ligand) Superfamily, Member 6] Locus

Abstract

A new polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis was developed for genetic typing of the mouse Tnfsf6^gld mutation. An artificial restriction site was introduced to the mouse Tnfsf6^gld mutation by PCR amplification using a modified primer. The three genotypes of the Tnfsf6 locus (Tnfsf6^gld/Tnfsf6^gld, Tnfsf6^gld/+, and +^Tnfsf6-gld/+^Tnfsf6-gld) could be distinguished clearly and easily. This PCR-RFLP analysis was found to be useful for the identification of the mouse Tnfsf6^gld mutation.