Effects of Pair Housing on Diurnal Rhythms of Heart Rate and Heart Rate Variability in Miniature Swine

Abstract

This study investigated the effects of pair housing on diurnal rhythms of heart rate and autonomic nervous activity in miniature swine. For this purpose, six adult Göttingen miniature swine were initially housed individually in an animal cage. Then, two of each swine were housed in a large cage together for 3 weeks. After that swine were separated into individual cages again. During this experimental procedure, electrocardiogram (ECG) was recorded with a Holter ECG recorder. Autonomic nervous activity was evaluated by power spectral analysis of heart rate variability. Heart rate and autonomic nervous activity clearly showed a diurnal rhythm in miniature swine housed in individual cages. When two swine were housed together, heart rate was significantly increased throughout the day and diurnal rhythm disappeared. Although these changes gradually recovered to basal levels, these parameters had not completely returned to basal levels even after 2 weeks. Heart rate was still higher than the initial level just after swine were re-housed in their own individual cages. Heart rate and autonomic nervous activity returned to basal levels about 2 weeks after re-housing. Further, heart rate in some swine decreased below their initial levels. These results suggest that it takes miniature swine at least 2 weeks to adapt to different circumstances. Furthermore, the power spectral analysis of heart rate variability can be used as a useful method in a study for answering controversial issues related to stress response.