Experimental Animals
Online ISSN : 1881-7122
Print ISSN : 1341-1357
ISSN-L : 0007-5124
Original
Fertility of Spermatozoa Cryopreserved with 2% Acetamide or Glycerol through Artificial Insemination in the Japanese White Rabbit
Yasushi OKUDAYasunari SEITAShin HISAMATSUShigenori SONOKIMasao SHINOToshio MASAOKATomo INOMATAShin-ichi KAMIJONaomi KASHIWAZAKI
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JOURNAL OPEN ACCESS

2007 Volume 56 Issue 1 Pages 29-34

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Abstract
The rabbit is considered to be a valuable laboratory animal. We compared 2% acetamide and glycerol as cryoprotectants in egg-yolk diluent for ejaculated Japanese white rabbit spermatozoa to improve sperm cryopreservation methods. Fertility through artificial insemination, forward progressive motility and plasma membrane integrity of the post-thaw spermatozoa were examined. The rates of forward progressive motility and plasma membrane integrity of the spermatozoa frozen with acetamide (27.1 ± 8.3% and 24.5 ± 6.5%) were significantly (P<0.05) higher than those of the spermatozoa frozen with glycerol (16.3 ± 10.9% and 14.3 ± 7.6%). Though there was no significant difference in the kindling rates, the litter size of females inseminated with spermatozoa frozen with acetamide (6.0 ± 1.1) were significantly (P<0.05) higher than those of spermatozoa frozen with glycerol (3.0 ± 0.4). The results indicate that 2% acetamide has a higher cryoprotective effect than 2% glycerol for sperm cryopreservation in the Japanese white rabbit.
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© 2007 Japanese Association for Laboratory Animal Science

This article is licensed under a Creative Commons [Attribution-NonCommercial-NoDerivatives 4.0 International] license.
https://creativecommons.org/licenses/by-nc-nd/4.0/
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