Spontaneous Adenocarcinoma Immunoreactive to Cyclooxygenase-2 and Transforming Growth Factor-β1 in the Buccal Salivary Gland of a Richardson's Ground Squirrel (Spermophilus richardsonii)

Abstract

The ground squirrel is used as an experimental animal because of its unique biological nature. A 3-year-old female Richardson's ground squirrel developed a mass, 1.5 cm in diameter, in the buccal mucosa. The mass consisted of neoplastic epithelial cells showing acinar, ductular, intraductal papillary, solid, and lobular growth patterns; the cells were immunoreactive to cytokeratin, cyclooxygenase-2 (a marker of malignancy) and TGF-β1. After resection, the tumor recurred with increased area having a solid or lobular pattern with little differentiation. This tumor was diagnosed as an adenocarcinoma arising from the buccal gland, the first case reported in the ground squirrel. A prominent desmoplastic reaction was present. The interstitial cells reacted to α-smooth muscle actin and vimentin, indicating a myofibroblastic nature, presumably induced by epithelial TGF-β1.