A Diagnostic Method for Pneumocystis carinii a Causative Agent of Pneumonia in Immunodeficient Rats

Kaori YABUUCHI; Masaru TAJIMA; Yuko KOTANI; Katsumi AIHARA; Aiko KAWASAKI; Shiro KANEKO; Yasuhiro TAKAGI; Sumiko KAWAI; Kyoko SHIOYA; Akira OKAMOTO; Soichiro KAGIYAMA; Tsutomu KUROSAWA

doi:10.1538/expanim.59.261

Note

A Diagnostic Method for Pneumocystis carinii a Causative Agent of Pneumonia in Immunodeficient Rats

Kaori YABUUCHI, Masaru TAJIMA, Yuko KOTANI, Katsumi AIHARA, Aiko KAWASAKI, Shiro KANEKO, Yasuhiro TAKAGI, Sumiko KAWAI, Kyoko SHIOYA, Akira OKAMOTO, Soichiro KAGIYAMA, Tsutomu KUROSAWA

著者情報

キーワード: immunochemistry, immunodeficient rat, Pneumocystis carinii

ジャーナルオープンアクセス

2010 年 59 巻 2 号 p. 261-267

DOI https://doi.org/10.1538/expanim.59.261

詳細

抄録

Immunodeficient animals are in demand in current biomedical research, and they contribute to medical progress. In Pneumocystis infections, a specific histological diagnostic tool may be immunochemistry (IC). However, it was recently reported that the antibody (3F6) was not suitable for detecting Pneumocystis in rats. We purchased another antibody [PNC007] from a commercial source for IC. We could detect positive signals at identical locations with IC and Toluidine blue O in lungs of infected rats. These results corresponded to the results obtained with PCR. We should study the relationship between unexpected positive signals seen in IC and trophic forms in lungs of infected rats. We could clinically diagnose pneumonia caused by Pneumocystis carinii with the diagnostic method we developed.

責任著者(Corresponding author)

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