Experimental Animals
Online ISSN : 1881-7122
Print ISSN : 1341-1357
ISSN-L : 0007-5124
Original
Impairment of α2-Macroglobulin Synthesis in Experimental Hepatopathic Rats Treated with Turpentine Oil
Takashi KuribayashiTesturo SeitaToshio HonjoShunsuke YamazakiEiichi MomotaniShizuo Yamamoto
著者情報
キーワード: α2M, acetaminophen, CCl4, hepatopathy, rat
ジャーナル フリー

2012 年 61 巻 2 号 p. 125-130

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抄録

The aim of this study was to investigate the synthesis of α2-macroglobulin (α2M) in hepatopathic rats injected with turpentine oil to induce acute inflammation. Hepatopathy was induced by oral administration of acetaminophen at a dose of 1 g/kg daily for 2 weeks or a 25% solution of carbon tetrachloride (CCl4) at 2 ml/kg body weight three times per week for 7 weeks. Acute inflammation was induced by intramuscular injection of turpentine oil at a dose of 1.0 ml/kg body weight. Serum concentrations of α2M were measured by enzyme-linked immunosorbent assay. Aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase and total protein differed significantly between acetaminophen or CCl4-induced hepatopathic rats and acetaminophen control (AA-control) or CCl4 control (CC-control) rats. Furthermore, pathological examination confirmed hepatopathy in rat livers. Peak serum concentrations and area under the time-concentration curve for α2M showed significant differences between hepatopathic rats and AA-control or CC-control rats. Thus, serum concentrations of α2M did not increase when compared with nontreated rats.

著者関連情報
© 2012 Japanese Association for Laboratory Animal Science
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