A Rat Model of Intracerebral Hemorrhage Permitting Hematoma Aspiration plus Intralesional Injection

Yan-Hua SANG; Yu-Xiang LIANG; Ling-Guang LIU; Rutledge G. ELLIS-BEHNKE; Wu-Tian WU; Kwok-Fai SO; Raymond Tak-Fai CHEUNG

doi:10.1538/expanim.62.63

Originals

A Rat Model of Intracerebral Hemorrhage Permitting Hematoma Aspiration plus Intralesional Injection

Yan-Hua SANG, Yu-Xiang LIANG, Ling-Guang LIU, Rutledge G. ELLIS-BEHNKE, Wu-Tian WU, Kwok-Fai SO, Raymond Tak-Fai CHEUNG

Author information

Yan-Hua SANG
Department of Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China
Department of Anatomy, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China
Yu-Xiang LIANG
Department of Anatomy, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China
State Key Laboratory of Brain and Cognitive Sciences, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China
Ling-Guang LIU
Department of Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China
Rutledge G. ELLIS-BEHNKE
Department of Anatomy, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China
State Key Laboratory of Brain and Cognitive Sciences, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China
Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, MA, USA
Department of Ophthalmology, Medical Faculty Mannheim, Ruprecht-Karls-University, Heidelberg, Germany
Wu-Tian WU
Department of Anatomy, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China
State Key Laboratory of Brain and Cognitive Sciences, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China
Research Centre of Reproduction, Development and Growth, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China
Kwok-Fai SO
Department of Anatomy, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China
State Key Laboratory of Brain and Cognitive Sciences, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China
Research Centre of Heart, Brain, Hormone and Healthy Aging, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China
Raymond Tak-Fai CHEUNG
Department of Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China
Research Centre of Heart, Brain, Hormone and Healthy Aging, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China

Keywords: animal model, hematoma aspiration, intracerebral hemorrhage, intralesional injection, rat

JOURNAL OPEN ACCESS

2013 Volume 62 Issue 1 Pages 63-69

DOI https://doi.org/10.1538/expanim.62.63

Details

Abstract

A combination of hematoma aspiration and local delivery of chemicals may be more effective than either therapy in intracerebral hemorrhage (ICH). The aim of the present study was to develop a rat model of hematoma aspiration plus intralesional injection after ICH. ICH was induced in adult Sprague-Dawley rats by an intrastriatal injection of bacterial collagenase IV. Hematoma aspiration was performed 3.5 h after ICH onset. Following aspiration, normal saline was injected into the lesion cavity. Hematoma aspiration with or without subsequent saline injection significantly reduced the hematoma volume, lesion volume, and perihematomal neutrophil infiltration. Hematoma aspiration plus subsequent intralesional injection is simple, feasible, and safe. This ICH model can be used to assess the effectiveness of hematoma removal plus local delivery of chemicals.

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