Evaluation of a TPTX model induced by ischemia

Abstract

Parathyroidectomy (PTX), especially total parathyroidectomy (TPTX), is often recommended for severe secondary hyperparathyroidism (SHPT) if other medical treatments fail. Accurate identification and resection of parathyroid gland (PTG) tissue is the cornerstone of PTX. The establishment of a rat TPTX model would be beneficial for several applications but faces the same problems. In this experiment, we studied the mechanisms of ischemia for the accurate identification and excision of PTG tissue to establish TPTX rat models and to analyze the effects of surgical removal of PTG tissue as well as the effects of different types of water intake in rats on clinical indices. We found that the ischemia method had advantages when establishing a rat TPTX model. Removal of the PTG tissue resulted in significantly changed postoperative indices, and varying the types of water intake induced significant differences in these indices after removal of the PTG tissue. The absolute value of the difference between the serum calcium and phosphorus concentrations (|Ca−P|) accurately reflected the effect of removal of the PTG tissue and was superior to the calcium-phosphorus product (Ca × P); Ca × P accurately reflected the effect of varying the types of water intake in rats and was superior to the |Ca−P|.