Experimental Animals
Online ISSN : 1881-7122
Print ISSN : 1341-1357
ISSN-L : 0007-5124
Original
CRISPR-Cas9-mediated generation of obese and diabetic mouse models
Jae-il RohJunghoon LeeSeong Uk ParkYoung-Shin KangJaehoon LeeAh-Reum OhDong Joon ChoiJi-Young ChaHan-Woong Lee
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JOURNALS FREE ACCESS

2018 Volume 67 Issue 2 Pages 229-237

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Abstract

Mouse models of obesity (ob/ob) and diabetes (db/db) in which the leptin (Lep) and leptin receptor (Lepr) genes have been mutated, respectively, have contributed to a better understanding of human obesity and type 2 diabetes and to the prevention, diagnosis, and treatment of these metabolic diseases. In this study, we report the first CRISPR-Cas9-induced Lep and Lepr knockout (KO) mouse models by co-microinjection of Cas9 mRNA and sgRNAs that specifically targeted Lep or Lepr in C57BL/6J embryos. Our newly established Lep and Lepr KO mouse models showed phenotypic disorders nearly identical to those found in ob/ob and db/db mice, such as an increase in body weight, hyperglycemia, and hepatic steatosis. Thus, Cas9-generated Lep and Lepr KO mouse lines will be easier for genotyping, to maintain the lines, and to use for future obesity and diabetes research.

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© 2018 Japanese Association for Laboratory Animal Science
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