Experimental Animals
Online ISSN : 1881-7122
Print ISSN : 1341-1357
ISSN-L : 0007-5124
Original
Pathological characteristics of Ccdc85c knockout rats: a rat model of genetic hydrocephalus
Shizuka KonishiNatsuki TanakaTomoji MashimoTakashi YamamotoTetsushi SakumaTakehito KanekoMiyuu TanakaTakeshi IzawaJyoji YamateMitsuru Kuwamura
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JOURNALS OPEN ACCESS

2020 Volume 69 Issue 1 Pages 26-33

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Abstract

Spontaneous hhy mice show hydrocephalus and subcortical heterotopia, and a mutation in the Ccdc85c gene has been identified. To contribute to the comparison of the role of Ccdc85c in different species, we established a Ccdc85c KO rat and investigated its pathological phenotypes. Ccdc85c KO rats were produced by genomic engineering using transcription activator-like effector nuclease (TALEN). The KO rats had an approximately 350-bp deletion in Ccdc85c and lacked CCDC85C protein expression. The KO rats showed non-obstructive hydrocephalus, subcortical heterotopia, and intracranial hemorrhage. The KO rats had many pathological characteristics similar to those in hhy mice. These results indicate that CCDC85C plays an important role in cerebral development in rats, and the function of CCDC85C in the cerebrum are similar in rats and mice.

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© 2020 Japanese Association for Laboratory Animal Science
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