Various biological functions of carbohydrate chains learned from glycosyltransferase-deficient mice

Abstract

Carbohydrate chains are attached to various proteins and lipids and modify their functions. The complex structures of carbohydrate chains, which have various biological functions, are involved not only in regulating protein conformation, transport, and stability but also in cell–cell and cell–matrix interactions. These functional carbohydrate structures are designated as “glyco-codes.” Carbohydrate chains are constructed through complex reactions of glycosyltransferases, glycosidases, nucleotide-sugar donors, and protein and lipid substrates in a cell. To elucidate the functions of carbohydrate chains, I and my colleagues generated and characterized knockout (KO) mice of galactosyltransferase family genes. In this review, I introduce our studies about galactosyltransferase family genes together with related studies performed by other researchers, which I presented in my award lecture for the Ando-Tajima Prize of the Japanese Association for Laboratory Animal Science (JALAS) in 2019.