Abstract
Purpose: We have recently reported that DNA fragmentation without cell loss were found in the hippocampus in EL mice, an epileptic mutant of epilepsy. And abundance of neurotrophic factors were expressed during the early developmental stage.
In the present study, we used the EL mice to examine how the altered cyclin and the corresponding Cyclin Dependent Kinases (CDK) family are related to the cell proliferation during development, and further the epileptogenesis.
Methods: Developmental changes of cyclin family and corresponding CDK were examined during cell cycle by Western blotting in the hippocampus of EL mice. Also, an attempt was made to identify the cell proliferation by using systemic Bromodeoxy Uridine (BrdU) to labell dividing cells.
Results: As compared with the control DDY, EL shows up-regulation of each cell cycle specific Cyclins/CDK's during early developmental stage which suggests that reentry of cell cycle is promoted before the beginning of seizures, possibly because of the abundance of neurotrophic factors.
Conclusions: It is concluded that the activation of Cyclins/CDK's during early developmental stage before exhibiting seizures, suggesting the reentry of cell cycle and cell proliferation in the hippocampus of EL.
