Application of STH-PAS, a novel chromatographic visualization system of PCR products for rapid screening of male-sterile lines in Cryptomeria japonica

Abstract

To construct a simple method for the genotyping MALE STERILITY 1 (MS1), a major causative gene of male sterility in Japanese cedar (Cryptomeria japonica), this study attempted to reduce the labor required for the postPCR procedure using a single-stranded tag hybridization chromatographic printed array strip (STH-PAS), which allows for visual confirmation of PCR products. PCR was performed using tagged DNA- or biotin-modified primers to develop amplification products on stick membranes with complementary tags. After ~15 min the detection line was colored and the MS1 genotype was determined. The diagnostic results were consistent with the electrophoresis results. STH-PAS can significantly reduce the time and labor required for MS1 determination.