Abstract
Various saccharification and fermentation conditions were examined to produce bioethanol efficiently from cotton. These conditions indicate liquor-to-sample ratios, amount of cellulase addition, amount of acetic acid buffer solution and the pH of acetic acid buffer solution. These conditions were varied within the range of practical conditions which can be achieved for a dyeing equipment. Furthermore, we adopted room temperature and short time alkali soaking as a pretreatment method. This method also can be achieved for a dyeing equipment. As a result of examinations, we got an optimized condition that was pH 5.0, 20% of cellulase addition for cotton's weight and we also cleared the relationship between liquor-to-sample ratios and obtained glucose concentration through a saccharification. The efficiency of ethanol fermentation was found to be 66-75% of theoretical yield.
