繊維にキレート結合を導入する研究
第15報 タン白質の重金属塩処理と赤外線吸収スペクトル
1966 年 22 巻 12 号 p. 527-531
詳細
1966 年 22 巻 12 号 p. 527-531
The effects of the treatments of silk fibroin with heavy metallic salts on absorbability in infrared spectra were studied. Silk fibroin was dissolved in an aqueous solution of LiBr, and the LiBr was removed by dialysis. The fibroin solutions treated with heavy metallic salts under various conditions were dialysed to remove the surplus reagents, poured on polystryrene film and dried at 40°C.
All the film of the fibroin thus obtained were found to absorb at 3300, 1660, and 1550 cm-1 in the spectra. Each optical density at 3300, 1660, and 1550cm-1 was shown as DI, DII and DIII respectively and relative densities D I/DII=(A) and DIII/DII=(B) were calculated.
The relative densities of original fibroin film DI0/DII0=(A0) and DIII0/DII0=(B0) were also taken. The values of A/A0 and B/B0 were plotted against atomic numbers and electronegativities. Most of the treated films showed lower values of A/A0 than I, and higher values of B/B0 than I, excepting those of treated with zinc and aluminium salts. These facts can be accounted for by considering that hydrogen bond at 3300cm-1 has been destroyed and C=O has been attacked by metallic salts. Optimum conditions are seemed to exist in the reactions of silk fibroin and metallic salts. It would be interesting to note that LiBr which is known as a powerful solvent of silk fibroin reacts with N-H and C=O but probably disturbs hydrogen bond very little.
