1984 Volume 40 Issue 9 Pages T317-T321
Thermal stability of invertase in aqueous solutions and immobilized in the films of two kinds of the photocrosslinked poly (vinyl alcohol) s, one of which additionally contained trimethylanilinium group, was studied calorimetrically. Calorimetric data showed that in aqueous solution, native invertase had an oligomeric structure. The denaturation temperature of invertase was raised and denaturation enthalpy was decreased by immobilization. The denaturation temperature of immobilized invertase, as well as in aqueous solution, was strongly affected by the pH of the solution into which sample films were dipped. The stabilizing effect can be explained largely by the effect of high concentration of invertase in the networks of PVA film.
