Lipolytic Behavior of Alkaline Lipase Observed for Fabric Surface and Aqueous Medium with or without SDS Surfactant

抄録

This study deals with an alkaline lipase, Lipolase, which is active in a wide pH range and is stable under alkaline conditions with considerable thermostability. The lipolytic behavior was observed both for fabric and the incubation media with a special attention to the effect of surfactant on the lipolysis. In the surfactant free media, lower melting triolein and trilaurin migrated partly from the surface of the fabric to the medium solutions and lipolysis proceeded both on the fabric surface and in the media. The hydrolysis of tripalmitin, however, proceeded only on the fabric. Concerning the ratios of residual triglycerides (TGs) and lipolytic products, fatty acids (FAs), Lipolase showed, as compared with Fusarium, higher ratios of more easily removable FAs and lower ratios of TGs, most stubborn lipid soil, indicating that Lipolase will act more advantageously in the washing system. In the presence of SDS, much more amounts of TGs, especially triolein, migrated to the media even without lipase. The inhibiting action of surfactant on lipolysis was proved by higher ratios of substrate TGs and lower ratios of hydrolyzate FAs detected both on the fabric and in the medium solutions.