キトサンのN-トリメチルアンモニウム塩の調製とその抗菌性
1994 年 50 巻 5 号 p. 215-220
詳細
1994 年 50 巻 5 号 p. 215-220
Three kinds of N-trimethylammonium salts of chitosan were prepared in order to study the antibacterial activities against Escherichia coli, ATCC 25922, (E. coli) and Staphylococcus epidermidis ATCC 149909, (St. epidermidis). N-Trimethylammonium iodide of chitosan (TMC-I) was prepared by the reaction of chitosan with methyl iodide. N-Trimethylammonium chloride of chitosan (TMC-C1) was prepared from TMC-I by exchanging on resin (Amberlite IRA-400 (C1-)) subsequently. N-Trimethylammonium hydroxide of chitosan (TMC-OH) was prepared from TMC-I by treating with aqueous NaOH solution and dialyzing. TMC-I and TMC-C1 became water soluble. TMC-OH was insoluble in water. The wide-angle X-ray diffraction patterns showed that TMC-I and TMC-C1 were amorphous and that TMC-OH was crystalline. TMC-I, TMC-CI and TMC-OH showed antibacterial activities against E. coli and St. epidermidis.
