抄録
The strain Col-B isolated from environment was found to be the most effective microorganism for the biosynthesis of violaceins as valuable natural pigments in the fermentation technique using waste wool. Col-B was classified as Chromobacterium violaceum. This technology is very important and useful from the point of recycle of waste wool which was produced in large amounts in wool industry. The yields of violaceins depended on the fermentation substrate including wool powder, Vitamin L1 and other additives, and the optimized fermentation conditions and compositions of substrates were determined for mass production of the pigments. Some other microorganisms which produced other natural pigments were also isolated in this project and their applications for the biosynthesis of pigments were under investigation. Violacein has strong biological activity against Staphylococcus aureus and antioxydant activity comparable to vitamin E, but deoxyviolacein produced together with violacein does not have such activities. Large scale biosynthesis using Col-B, waste wool and Vitamin L1 can be applied for practical synthesis of violacein pigments in commercial scale.
