Abstract
12-Hydroxyeicosatetraenoic acid and 15-hydroxyeicosatetraenoic acid were produced as major and minor monohydroxylated products in a microsome fraction, when [1-14C]arachidonic acid was incubated with the microsome or cytosol fraction prepared from frozen stored gill tissue of red sea bream Pagrus major. The endogenousproducts extracted from the microsome fraction of red sea bream gill were isolatedby high performance liquid chromatography and identified as 12-hydroxyeicosatetraenoic acid, 12-hydroxyeicosapentaenoic acid and 14-hydroxydocosahexaenoic acid by ultraviolet absorption spectrometry and gas chromatography-mass spectrometry.These data suggest that arachidonic acid, eicosapentaenoic acid and docosahexaenoic acid are converted to their monohydroxy derivatives via the hydroperoxides bythe action of 12-lipoxygenase-like enzyme, which is distributed in the microsomesof red sea bream gill.
