Application of Isoelectric Focusing in Thin-Layer Polyacrylamide Gels to the Study of Opine Dehydrogenases in Marine Invertebrates

Nobuhiro Kanno; Kunihide Kamimura; Eizou Nagahisa; Minoru Sato; Yoshikazu Sato

doi:10.2331/fishsci.62.122

Nobuhiro Kanno, Kunihide Kamimura, Eizou Nagahisa, Minoru Sato, Yoshikazu Sato

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Keywords: isoelectric focusing, opine dehydrogenase, distribution, substrate specificity, multiple enzyme forms, opine

JOURNAL FREE ACCESS

1996 Volume 62 Issue 1 Pages 122-125

DOI https://doi.org/10.2331/fishsci.62.122

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Abstract

Application of isoelectric focusing in thin-layer polyacrylamide gels (IEF/PAG) to the study of opine dehydrogenases in marine invertebrates was described.The optimized IEF/PAG and the following activity bands detection showed high resolution and good reproducibility.It was useful for the detection of multi-functional enzymes which metabolize two (or more) distinct opines, and for the analysis of multiple enzyme forms (allozymes and isoenzymes) in tissues of marine invertebrates.

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