Abstract
HCc is a major basic nuclear protein of Crypthecodinium cohnii (Dinophyceae). Inorder to elucidate the gene organization of HCcl gene, it was amplified by PCR methods using the total DNA as atemplate and the nucleotide sequences of HCcl gene and its flanking regions wereestablished. Theresults were as follows: [1] HCcl gene was composed of multiple copies and arranged in tandem; [2] itcontained 4 introns lacking the GT-AG consensus sequences, which are conserved inother eukaryoticintrons; and [3] eukaryotic promoter consensus sequences such as TATA box or CAAT box were not observed in the flanking regions of HCcl gene. An interesting pointis whether such atypical-eukaryotic features of the HCcl gene organization are common in dinoflagellates or not.
