Abstract
The laboratory stock culture of Chattonella marina harbors several bacterial stains and has been surviving in hundred times of transfer process. As predominant bacteria, Alteromonas, Flexibacter and Pseudomonas spp. were isolated from a non-axenic culture of C. marina. In the stateof mixed cultures of an axenic algal strain with co-existent bacterial isolates, the living algal cells were maintained over 30 days after transferred to enrichment sea water (ESS) medium. On the other hand, algal cells began to decrease at 10 days and were not detected at 30 days at all inaxenic cultures. On the addition of enrichment solution (ESP) into axenic cultures, algal cells were stimulated to grow evenafter reached maximum growth. When above 100mg/l of polypepton was added to non-axenic cultures, the algal cellcounts decreased as the total bacterialcounts increased. If the supernatant solutionsof co-existent bacterial cultures incubated in nutrient rich media (ZE-CI) were added to algal axenic cultures, algalcells were completely killed in above 5% concentrations within 7 days incubation.
