1999 Volume 65 Issue 4 Pages 558-562
Methane-oxidizing bacteria (methanotrophs) were isolated from sediments of estuaries. The representative strains, GM-1 and NM-11, are gram negative motile rods and exhibit characteristics typical of Type I methanotrophs. Both strains possessed cytomembranes arranged in stacked lamellae, and their predominant fatty acids were C16:1 and C16:0. The DNA base ratios of the strains, GM-1 and NM-11, were 51.0, 52.9% mol G+C, respectively. The strains require NaCI, grew well on methane or methanol, but not on any other compounds as a cabon source, and their growth was inhibited in natural light.