Comparison of characteristics of actomyosin from white, pink, and red muscle fiber types in cultured carp

Abstract

The present study compared and examined the characteristics of actomyosin among white (W), pink (P), and red (R) muscle fiber types in carp (cultured). Both the superprecipitation reaction and the Mg²⁺-ATPase activity of actomyosin became higher with increased Ca²⁺ concentration (pCa 7.0-pCa 5.0) and with decreased adenosine triphosphate (ATP) concentration (3.0-0.5 mM) in all three muscle fiber types. A comparison of the three fiber types shows that the superprecipitation reaction of actomysoin was lower in the order of W<P<R and, in contrast, was higher for Mg²⁺-ATPase activity in the order of W>P>R. A significantly positive correlation between both values was found for each of the three muscle fiber types, but these correlations were clearly different among the three muscle fiber types, and the superprecipitation reaction of actomyosin was lower in the order of W<P<R when Mg²⁺-ATPase activity was at the same level. In conclusion, the characteristics of actomyosin were remarkably different among white, pink, and red muscle fiber types.