2002 Volume 68 Issue sup2 Pages 1087-1090
Species-specific markers of three commercially cultured oysters (Crassostrea belcheri, C. iredalei and Saccostrea cucullata) in Thailand were identified by RAPD-PCR and PCR-RFLP analyses. Two hundred and fifty-four RAPD fragments were generated across overall samples in this study. High levels of genetic polymorphism were observed in Thai oysters. Ten, five and two species-diagnostic RAPD markers were found in C. belcheri, C. iredalei and S. cucullata, respectively. In addition, restriction analysis of the amplified COI gene segment with Acs I, Dde I and Mbo I provided 6, 13 and 22 restriction patterns, respectively. Species-specific RFLP patterns were also found in those oysters. A molecular taxonomic key based on RAPD and RFLP markers was constructed and successfully used for identification of the species origins of C. belched, C. iredalei and S. cucullata in Thailand
