Pharmacological Studies on the Glucose Mobilization of Toad's Liver

Report I

Abstract

It is a well-known fact that the glucose is produced in the perfusing solution of toad's liver when perfused with normal Ringer's solution which originally did not contain any trace of glucose. This phenomenon is known as the glucose mobilization " of liver. A numerous articles have already been published for determination of the funktion of carbohydrate metabolism in toad's liver by perfusing with Ringer's solution which is an established method that now generally in practice in this field of study. I observed, through my experiments, quite accurately a seasonal change and also the rate of chemical component of the perfusing solution that affects on this glucose mobilization.
The results are as follows : 1) Glucose mobilization of toad's liver in normal condition is more variable by an individuality of the animal than by their change of body weight or by that of weight of liver. 2) When the Ringer's solution without glucose was perfused, it was found makedly affected by seasons. Thus, it shows 0.016-0.022 mg/dl in hinbernating stage, whilst it showed 0.023-0.028 mg/dl during in their active stage. Therefore, 1 in such way determined some fluctuation of the value of glucose mobilization and which was compared with that of the normal value in the season. 3) When a different concentration of glucose in Ringer's solution was perfused, it showed a corresponding decrease of glucose mobilization of the liver proportionally with the concentration of the glucose that had been administered. 4) When the solution contaifling only sodium bicarbonate and sodium chloride was perfused, it showed no difference from the Ringer's solution of that without glucose. The observation on influence of the glucose mobilization by giving concentration of the calcium chloride and potasium chloride in certain ratio, shows that it was promoted by calcium chloride (0.003-0.1 %), while inhibited by potasium chloride (0.003-0.3 %). 5) When perfused with the Ringer's solution without glugose which was buffered with phosphate (free from sodium bicarbonate), the influence on the glucose mobilization was augmented by an increase of the ph and inhibited by its decrease. 6) Glucose mobilization was not markedly influenced by the temperature of the perfusing solution. [Vgl. Original (japanisch) S. 521.]