Fundamental Toxicological Sciences
Online ISSN : 2189-115X
ISSN-L : 2189-115X
Toxicomics Report
Proteomic analysis of valproic acid-induced embryotoxicity in cultured post-implantation rat embryos
Makoto UsamiMina TakamatsuShugo KazamaKatsuyoshi MitsunagaAtsuko MiyajimaTomohiko IrieOsamu DoiTatsuya TakizawaTeruko NagiMomoko Sunouchi
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2017 Volume 4 Issue 1 Pages 31-35


Protein expression changes were examined in day 10.5 rat embryos cultured for 24 hr in the presence of valproic acid (VPA), using two-dimensional electrophoresis and mass spectrometry. Exposure to VPA at an embryotoxic concentration of 1.2 mM resulted in quantitative changes in many embryonic protein spots (22 decreased and 29 increased). For the increased protein spots, 10 proteins were identified, including alpha-fetoprotein, phosphorylated cofilin-1, and serum albumin. These proteins are candidate protein biomarkers that may be involved in embryotoxic mechanisms.

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© 2017 The Japanese Society of Toxicology
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