Abstract
This is a preliminary report on the endoscopic dyeing method using by crystal violet. The crystal violet is a violet color dye with pH 3.2, but it changes into violet, blue, green and yellow colores respectively by gradient pH solution from pH3.2 to pH 0.15. In order to measure the pH of the surface of the gastric mucosa, we carried out in vitro experiments with gradient artificial gastric juice and endoscopical examination by crystal violet solution. We performed clinical experiments by using three methods; (1) crystal violet solution was scattered on the gastric mucosal surface after the conventional endoscopic examination. (2) tetra-gastrin 4γ/kg i.m. and 50 ml of 0.5% NaHCO3 solution p.o. were administered, after 15 minutes a conventional endoscopic examination was performed, then, crystal violet solution was scattered on the gastric mucosal surface endoscopically. (3) the method was similar to (2) but.100 ml of 5% NaHCO3 solution was administered. Results; We found that the atrophic gastritis and pyloric gland regions were stained violet with crystal violet solution and fundic gland region was not stained but irregularities of mucosal surface were stained blue in all the three method. This blue color of crystal violet appears at the pH range from 2.7 to 1.7 in vitro. In some cases mucosal surface was stained green in color in the 2nd method. This green color of crystal violet solution appears at the pH range from 1.5 to 0.7 in vitro On close observation of stained regions with crystal violet solution, minute surface structure of gastric mucosa were visualized by conventional endoscopy. Conclusions; Endoscopic dyeing method by crystal violet solution demonstrated that pH of gastric mucosal surface is endoscopically visible and the endoscopic atrophic border were clearly demonstrated by the use of crystal violet, because of stain of the atrophic gastritis and pyloric gland regions, especially, on close observation, minute mucosal surface structures were visible by conventional endoscope and the irregularities on the surface of the fundic gland region were clearly demonstrated by the use of crystal violet dye. From these results it is apparent that our present new method of crystal violet dyeing endoscopy is very simple, easy and effective method for the physiological and morphological diagnosis of the gastric mucosal surface.
