Abstract
The ulcerative disease of colon was made in rabbit by the oral administration of 1% aqueous solution of amylopectin sulfate. The agent was given intermittently, that was, rabbit was fed amylopectin sulfate for two days and then received water without the agent for five days. This cycle of feeding was repeated many times for 6 weeks maximally. After two times of the repetition, ulcerative lesion was indused in 17% of the rabbits used in this experiment. The endoscopic dye scattering method made possible to show abnormal patterns of mucosal surface. Dissecting microscopic specimen revealed irregurarity of colonic mucosal area and irregular arrangement of cryptal openings. Scanning electron microscopic findings showed disappearance of cell border surrounding cryptal openings, loss of microvilli and transformation of microvilli like polypoid or hair shapes. After 4 or 6 times of the repetition, a macroscopic ulcerative lesion was induced in 67% of the rebbits used in this experiment. The ulcerative lesion made in this experiment was histopathologically very similar to ulcerative colitis in man. Endoscopic examination revealed petechiae, erosions and rough mucosal surface diffusely. In some cases the dissecting microscopic study revealed the disappearance of colonic mucosal area. Scanning electron microscopic study showed severe changes of colonic mucosa, destruction and a decrease in cryptal openings and a further decrease in number of microvilli. These findings were very significant considering the similar ones in ulcerative colitis in man.
