Abstract
This research reports method development for lipid peroxide measurement in natural waters. A novel fluorescent probe 2-(4-diphenylphosphanyl-phenyl) -9-(3,6,9,12-tetraoxatridecyl)-anthra[2,1,9-def:6,5,10-d'e'f']diisoquinoline-1,3,8,10-tetraone, Liperfluo, has been used to selectively quantify lipid peroxide in seawater. A Flow injection analysis (FIA) system equipped with fluorescence detector was designed and used to obtain fluorescence data. This method was observed to be highly selective to lipid hydroperoxide as the signal intensity from the reaction of the probe with lipid hydroperoxide in water was about 28-times higher than the signal intensity obtained upon reacting with the same concentrations of other peroxides such as Hydrogen peroxide, Methyl Hydroperoxide and Ethyl hydroperoxide present in natural waters.. Under our experimental conditions, the concentrations of lipid hydroperoxide in Seto inland sea ranged from 96.21 - 382 nM.
