Abstract
Age and sex related changes of the γ-carboxyglutamic acid (Gla) content in the rat bone was examined γ-carboxyglutamic acid in the rat femur, tibia, humerus and calvaria was measured by high pressure liquid chromatography. Gla content in each bone was significantly greater in the shaft than in the epiphysis.
It was found that Gla content in each bone significantly increased with advancing age and at 3 and 6 months of age, Gla content was significantly greater in the male than in the female. Significant positive correlation was found between Gla content and calcium and phorphorus in the bones respectively. These findings suggest the role of Gla in the mineralization of the bone.
